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Application of the BioFire FilmArray Blood Culture Identification Panel for Rapid Identification of the Causative Agents of Ventilator Associated Pneumonia.
Clin Microbiol Infect. 2018 Jun 12;:
Authors: Pulido MR, González-Galán V, Fernández Cuenca F, Pascual Á, Garnacho-Montero J, Antonelli M, Dimopoulos G, Lepe JA, McConnell MJ, Miguel Cisneros J, Magic Bullet Working Group
Abstract
OBJECTIVE: The objective of this study was to evaluate the ability of the BioFire FilmArray Blood Culture Identification (BCID) Panel to rapidly detect pathogens producing late-onset ventilator-associated pneumonia (VAP), a severe infection often produced by Gram negative bacteria. These microorganisms are frequently multidrug resistant (MDR) and typically require broad spectrum empiric treatment.
METHODS: In this study, in the context of an international multicenter clinical trial (Magic Bullet), respiratory samples were collected at the time of suspicion of VAP from 165 patients in 32 participating hospitals in Spain, Greece and Italy. Microorganisms were identified using the BCID Panel and compared with results obtained by conventional microbiologic techniques.
RESULTS: Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae were the most commonly identified species, representing 54.7% (70/128) of microorganisms. The BCID Panel showed high global specificity (98.1%; 95% CI = 96%-100%) and negative predictive values (96.6%) and a global sensitivity and positive predictive value of 78.6% (95% CI = 70%-88%) and 87.3%, respectively for these microorganisms. Importantly, the BCID Panel provided results in only one hour directly from respiratory samples with minimal sample processing times.
CONCLUSIONS: The BCID panel may have clinical utility in rapidly ruling out microorganisms causing VAP, specifically MDR-Gram negative species. This could facilitate the optimization of empiric treatment.
PMID: 29906599 [PubMed - as supplied by publisher]